Sperm retrieval techniques for assisted reproduction

Sperm retrieval techniques for assisted reproduction.

Different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE have been developed to retrieve spermatozoa from either the epididymis or the testis according to the type of azoospermia, i.e., obstructive or non-obstructive. Laboratory techniques are used to remove contaminants, cellular debris, and red blood cells following collection of the epididymal fluid or testicular tissue. Surg...

Variation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques

Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal of the present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect sperm DNA integrity and impact pregnancy rate (PR), we prospectively evaluated sperm DNA fragmentation (sDF) by TUNEL/PI, before and after DG...

Profound Transcriptomic Differences Found between Sperm Samples from Sperm Donors vs. Patients Undergoing Assisted Reproduction Techniques Tends to Disappear after Swim-up Sperm Preparation Technique

Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up process in control donors and infertile males with the purpose to identify their functional significance in male fertility. MaterialsAndMethod...

Ethical issues in assisted reproduction techniques for patients with disorders of sexual development

Use of Assisted Reproduction Techniques in Male Pathologies: Molecule Based Sperm Selection Methods

Submit Manuscript | http://medcraveonline.com Abbreviations: WHO: World Health Organization; ROS: Reactive Oxygen Species; PS: Phosphatidylserine; EPS: Externalization of Phosphatidylserine; ART: Assisted Reproduction Techniques; IUI: Intra-Uterine Insemination; IVF: In Vitro Fertilization; ICSI: Intracytoplasmic Sperm Injection; PICSI: Physiologic Intracytoplasmic Sperm Injection; SU: Swim-Up;...